ONTOLOGY SOURCE REFERENCE
Term Source Name	OBI	EFO	UO	NCBITaxon	PO	GMI_accessions
Term Source File	http://data.bioontology.org/ontologies/OBI	http://data.bioontology.org/ontologies/EFO	http://purl.obolibrary.org/obo/UO	http://data.bioontology.org/ontologies/NCBITAXON	http://data.bioontology.org/ontologies/PO	http://gwas.gmi.oeaw.ac.at/#/taxonomy/1/passports?alleleAssayId=0
Term Source Version	23	118		6	10
Term Source Description	Ontology for Biomedical Investigations	Experimental Factor Ontology	Unit Ontology	National Center for Biotechnology Information (NCBI) Organismal Classification	Plant Ontology	Cataloque of Arabidopsis accessions at GMI
INVESTIGATION
Investigation Identifier	study36
Investigation Title	Salt induced changes in Root System Architecture
Investigation Description	Salt stress affects not only plant size, but also plant architecture. In this study, we focused on salt stress-induced changes to Root System Architecture, by germinating the seedlings on vertical agar growth medium (no salt) and transplanting 4 days old seedlings into plates containing 0, 75 and 125 mM NaCl. The Root System Architecture phenotypes were quantified using EZ-Rhizo software for at least 4 replicates per accession per condition. The plants grown under control conditions were measured 4 days after transfer (8 days after germination), while plants growing at both salt stress conditions were measured 8 days after transfer (12 days after germination). The Root System Architecture traits include Main Root Length, Total Root Size, Lateral Root Length and ~ Density, as well as the length of individual zones (basal, branching, apical) on the main root. 

The results of this study were published as Julkowska et al., Plant Cell, 2018, https://doi.org/10.1105/tpc.16.00680
Investigation Submission Date	2025-11-11 18:41:21.305060
Investigation Public Release Date	2025-11-11 18:41:21.305067
Comment [Created with configuration]    isaconfig-phenotyping-basic
Comment [Last Opened With Configuration]	isaconfig-phenotyping-basic
INVESTIGATION PUBLICATIONS
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STUDY
Study Identifier	study36
Study Title	Salt induced changes in Root System Architecture
Study Description	
Study Submission Date	2025-11-11 18:41:21.307435
Study Public Release Date	2025-11-11 18:41:21.307437
Study File Name	s_study36.txt
STUDY DESIGN DESCRIPTORS
Study Design Type	Salt stress affects not only plant size, but also plant architecture. In this study, we focused on salt stress-induced changes to Root System Architecture, by germinating the seedlings on vertical agar growth medium (no salt) and transplanting 4 days old seedlings into plates containing 0, 75 and 125 mM NaCl. The Root System Architecture phenotypes were quantified using EZ-Rhizo software for at least 4 replicates per accession per condition. The plants grown under control conditions were measured 4 days after transfer (8 days after germination), while plants growing at both salt stress conditions were measured 8 days after transfer (12 days after germination). The Root System Architecture traits include Main Root Length, Total Root Size, Lateral Root Length and ~ Density, as well as the length of individual zones (basal, branching, apical) on the main root. 

The results of this study were published as Julkowska et al., Plant Cell, 2018, https://doi.org/10.1105/tpc.16.00680
Study Design Type Term Accession Number	
Study Design Type Term Source REF	
STUDY PUBLICATIONS
Study PubMed ID	29114015
Study Publication DOI	10.1105/tpc.16.00680
Study Publication Author List	Magdalena M. Julkowska, Iko T. Koevoets, Selena Mol, Huub Hoefsloot, Richard Feron, Mark A. Tester, Joost J.B. Keurentjes, Arthur Korte, Michel A. Haring, Gert-Jan de Boer, Christa Testerink
Study Publication Title	Magdalena M. Julkowska, Iko T. Koevoets, Selena Mol, Huub Hoefsloot, Richard Feron, Mark A. Tester, Joost J.B. Keurentjes, Arthur Korte, Michel A. Haring, Gert-Jan de Boer, Christa Testerink
Study Publication Status	published
Study Publication Status Term Accession Number	1796
Study Publication Status Term Source REF	EFO
Study FACTORS
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STUDY ASSAYS
Study Assay File Name	a_study36.txt
Study Assay Measurement Type	phenotyping
Study Assay Measurement Type Term Accession Number	23
Study Assay Measurement Type Term Source REF	OBI
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STUDY PROTOCOLS
Study Protocol Name	Data transformation
Study Protocol Type	Data transformation
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