| Flowering time in simulated seasons | Two independent experiments were conducted in this study. Experiment 1 was done with a mapping population of 360 accessions (33), and Experiment 2 used a set of 473 accessions. Each accession in each experiment had four replicates under each of the four growth conditions (two planting seasons by two locations).
Both experiments were conducted in two walk-in growth chambers (AR-916, Percival Scientific) that were programmed to cycle the local climates every 5 min from the simulated weather files. The simulated climates were generated by using SolarCalc (35) with sunrise and sunset, light spectrum, temperature, and relative humidity programmed to cycle throughout the day and the season according to 1975?2000 averages (Fig. S5). One chamber was simulating Spain (latitude 41.72091, longitude 2.957075) starting from March 1, and the other chamber was simulating Sweden (latitude 55.71226, longitude 13.207352) starting from May 1st on the first day when plants were put into the chambers. | 34 | Aug/13/2016 |
| Adaptive diversification of growth allometry in the plant Arabidopsis thaliana | The file contains average trait values per accession for plant life cycle duration ('LifeCyleDuration', days), total fruit number ('FruitNumber'), final rosette dry mass ('rosetteDM', mg), absolute growth rate ('GrowthRate', mg d-1), relative growth rate ('RGR', mg d-1 g-1), and the scaling exponent ('ScalingExponent').
Accessions are identified with their 1001-genomes IDs (http://1001genomes.org/). | 5 | Apr/03/2019 |
| 1001genomes flowering time phenotypes | Study of flowering time at 16°C (FT16) and flowering time at 10°C (FT10) that where phenotype for the 1001genomes project. | 2 | Aug/14/2016 |
| Salt induced changes in Root System Architecture | Salt stress affects not only plant size, but also plant architecture. In this study, we focused on salt stress-induced changes to Root System Architecture, by germinating the seedlings on vertical agar growth medium (no salt) and transplanting 4 days old seedlings into plates containing 0, 75 and 125 mM NaCl. The Root System Architecture phenotypes were quantified using EZ-Rhizo software for at least 4 replicates per accession per condition. The plants grown under control conditions were measured 4 days after transfer (8 days after germination), while plants growing at both salt stress conditions were measured 8 days after transfer (12 days after germination). The Root System Architecture traits include Main Root Length, Total Root Size, Lateral Root Length and ~ Density, as well as the length of individual zones (basal, branching, apical) on the main root.
The results of this study were published as Julkowska et al., Plant Cell, 2018, https://doi.org/10.1105/tpc.16.00680 | 59 | Jun/30/2019 |
| Abscisic acid (ABA) treatment | Ristova et al., (2018): Natural Genetic Variation Shapes Root System Responses to Phytohormones in Arabidopsis. Plant Journal. DOI: 10.1111/tpj.14034. | 9 | Jan/16/2019 |
| Cytokinin (CK) treatment | Ristova et al., (2018): Natural Genetic Variation Shapes Root System Responses to Phytohormones in Arabidopsis. Plant Journal. DOI: 10.1111/tpj.14034. | 9 | Jan/16/2019 |
| Auxin (IAA) treatment | Ristova et al., (2018): Natural Genetic Variation Shapes Root System Responses to Phytohormones in Arabidopsis. Plant Journal. DOI: 10.1111/tpj.14034. | 9 | Jan/16/2019 |
| Control (C) treatment | Ristova et al., (2018): Natural Genetic Variation Shapes Root System Responses to Phytohormones in Arabidopsis. Plant Journal. DOI: 10.1111/tpj.14034. | 9 | Jan/16/2019 |
| Raffinose (22°C and 32°C) | Raffinose measurements at 22°C and 32°C
published in Reichelt et al. 2023
https://doi.org/10.1101/2023.05.15.540763 | 2 | Jun/26/2023 |
| Ion Concentration | Plants were grown in a controlled environment with 10 h light/
14 h dark (90 mmol m22s21 photosynthetically active light) and 19
to 22uC, as previously described [31]. Briefly, seeds were sown
onto moist soil (Promix; Premier Horticulture) in 10.506210 20 row
trays with various elements added to the soil at subtoxic
concentrations (As, Cd, Co, Li, Ni, Rb, and Se [31]) and the
tray placed at 4uC for 3 days to stratify the seeds and help
synchronize germination. Each tray contained 108 plants, six
plants each from 18 accessions, with three plants of each accession
planted in two different parts of the tray. Each tray contained four
common accessions (Col-0, Cvi-0, Fab-2 and Ts-1) used as
controls, and 14 test accessions. Trays were bottom-watered twice
per week with 0.25-strength Hoagland solution in which Fe was
replaced with 10 mM Fe-HBED[N,N9-di(2-hydroxybenzyl)ethyle-
nediamine-N,N9-diacetic acid monohydrochloride hydrate; Strem
Chemicals, Inc.). After 5 weeks plants were non-destructively
sampled by removing one or two leaves and the elemental
composition of the tissue analyzed by Inductively Couple Plasma
Mass Spectroscopy (ICP-MS). The plant material was rinsed with
18 MV water and placed into Pyrex digestion tubes. | 19 | Mar/21/2017 |
| 1001 Genomes & easyGWAS | Phenotypes from the 1001 Genomes Project and the easyGWAS publication | 8 | Jun/26/2019 |
| Isoprenoid concentration | Natural variation of seven different isoprenoids in 118 different accessions | 7 | Oct/25/2020 |
| Natural variation in stomata size (Dittberner et al. 2018) | Measurements of stomata site, density and water use efficiency as described by Dittberner et al. 2018 in Molecular Ecology
DOI: 10.1111/mec.14838 | 6 | Jul/31/2019 |
| Satbhai et. al. Nature Communications 2017 | Low availability of Fe significantly limits crop yields in many parts of the world. However, it is largely unknown which genes and alleles adjust plant growth in Fe limited environments. Using natural variation of a geographically restricted panel of Arabidopsis thaliana accessions, we identify allelic variation at the FRO2 locus associated with root length under iron deficiency. We show that non-coding sequence variation at the FRO2 locus leads to variation of FRO2 transcript levels, as well as ferric chelate reductase activity, and is causal for a portion of the observed root length variation. These FRO2 allele dependent differences are coupled with altered seedling phenotypes grown on iron-limited soil. Overall, we show that these natural genetic variants of FRO2 tune its expression. These variants might be useful for improvement of agronomically relevant species under specific environmental conditions, such as in podzols or calcareous soils (Reference: doi:10.1038/ncomms15603). | 5 | Sep/05/2017 |
| Genetic dissection of shoot regeneration from root explants in Arabidopsis (Lardon et al., 2020) | In the frame of a genome-wide association study, we have subjected 170 natural Arabidopsis thaliana accessions to two protocol variants for shoot regeneration from root explants and recorded substantial variation in regenerated shoot numbers and several related in vitro traits. The results of this study are reported in "The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors" (Lardon et al., Commun. Biol., 2020). | 28 | Aug/20/2020 |
| Kerdaffrec et al. 2017 | Germination rate of seeds after-ripened for 21, 63 and 105 days measured for 92 Swedish lines grown under two different temperature treatments, cold (15ºC) and warm (21ºC). | 6 | Mar/15/2018 |
| Kerdaffrec et al. 2016 | Germination rate of seeds after-ripened for 21 days (GR21) measured for 161 Swedish lines. A more detailed description can be found in Kerdaffrec et al. 2016. | 1 | Mar/15/2018 |
| DAAR | GWAS of three mass features of N-malonyl-D-allo-isoleucine in Arabidopsis thaliana inbred lines using ~250k SNPs in 416 accessions | 3 | Sep/05/2017 |
| Atwell et. al, Nature 2010 | GWAS of 107 phenotypes in Arabidopsis thaliana inbred lines using ~250k SNPs in 199 accessions | 107 | Aug/13/2016 |
| Seed longevity (Renard et. al., 2020) | Four different seed aging treatments (3 artificial treatments (AAT, CDT and EPPO) and dry seed storage (NAT)) were scored in 269 Arabidopsis thaliana ecotypes. | 4 | Aug/26/2020 |
