Phenotype: shoot primordia 15d protocol b
ID:
1275
Study:
AraGWAS Associations:
Scoring:
Manual count of regenerated shoot primordia from root explants after 15 days on SIM following protocol b. Shoot primordia were defined as dome-shaped outgrowths with a purple or green colour and clearly organized cellular patterning, indicated by a smooth surface.
Growth conditions:
Protocol b: 7-mm-long apical root segments were excised from 10-day-old seedlings, grown under warm white fluorescent tungsten tubes (70 µM m-2 s-1; 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to shoot induction medium (SIM; B5 supplemented with 5 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid).
Trait-Ontology:
Environment ontology:
Unit:
Shapiro-Wilk*:
'0.00e+00' (p-value)
Submission date:
Aug. 18, 2020, 9:32 a.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 2164 accessions
Phenotype Publications
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The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors
Communications Biology. 2020 3: None. doi: https://doi.org/10.1038/s42003-020-01274-9