Phenotype: greening 15d protocol b
ID:
1280
Study:
AraGWAS Associations:
Scoring:
Greening of root explants after 15 days on SIM following protocol b. Greening was determined automatically in ImageJ as 255 minus the intensity of green values in the area of explants, obtained by subtracting the background with a colour threshold.
Growth conditions:
Protocol b: 7-mm-long apical root segments were excised from 10-day-old seedlings, grown under warm white fluorescent tungsten tubes (70 µM m-2 s-1; 14/10 h light/dark regime) at 21 °C. Next, explants were preincubated on callus induction medium (CIM; B5 supplemented with 2.2 µM 2,4-dichlorophenoxy acetic acid and 0.2 µM kinetin) for 4 days, before being transferred to shoot induction medium (SIM; B5 supplemented with 5 µM 2-isopentenyl adenine and 0.86 µM 3-indole acetic acid).
Trait-Ontology:
Environment ontology:
Shapiro-Wilk*:
'1.65e-38' (p-value)
Submission date:
Aug. 18, 2020, 9:32 a.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 2132 accessions
Phenotype Publications
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The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors
Communications Biology. 2020 3: None. doi: https://doi.org/10.1038/s42003-020-01274-9