3 g of fresh seedlings, supplemented with internal standards were homogenized in 20 ml of chloroform/methanol solution (1/1, v/v) and extracted for 24 h at 25 °C.
Plants were grown in a growth chamber in a long day (16 h light) photoperiod at 22 °C/18 °C at day/night. The seeds were surface-sterilized by treatment with an aqueous solution of 5% calcium hypochlorite for 8 min, subsequently rinsed four times with sterile water and planted on plates. Before location in the growth chamber, plates with seeds were kept for 4 days at 4 °C in darkness for stratification. The Arabidopsis accessions were grown on Petri dishes on solid ½ Murashige-Skoog medium with vitamins and 0.8% agar. For each genotype analyzed plants were cultivated in at least three biological replicates.
Oct. 25, 2020, 12:47 p.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.