Phenotype: Sterol
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ID:
1316
AraGWAS Associations:
Scoring:
3 g of fresh seedlings, supplemented with internal standards were homogenized in 20 ml of chloroform/methanol solution (1/1, v/v) and extracted for 24 h at 25 °C.
Growth conditions:
Plants were grown in a growth chamber in a long day (16 h light) photoperiod at 22 °C/18 °C at day/night. The seeds were surface-sterilized by treatment with an aqueous solution of 5% calcium hypochlorite for 8 min, subsequently rinsed four times with sterile water and planted on plates. Before location in the growth chamber, plates with seeds were kept for 4 days at 4 °C in darkness for stratification. The Arabidopsis accessions were grown on Petri dishes on solid ½ Murashige-Skoog medium with vitamins and 0.8% agar. For each genotype analyzed plants were cultivated in at least three biological replicates. For the isolation of isoprenoids, entire 3-week-old seedlings were used. 3 g of seedlings were homogenized in 6 ml ethanol and extracted for 24 h at 25 °C.
Environment ontology:
Shapiro-Wilk*:
'1.67e-11' (p-value)
Submission date:
Oct. 25, 2020, 12:47 p.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 114 accessions
Phenotype Publications
  • GWAS analysis combined with QTL mapping identify CPT3 and ABH as genes underlying dolichol accumulation in Arabidopsis

    Katarzyna Gawarecka, Joanna Siwinska, Jaroslaw Poznanski, Agnieszka Onysk, Przemyslaw Surowiecki, Liliana Surmacz, Ji Hoon Ahn, Arthur Korte, Ewa Swiezewska, Anna Ihnatowicz

    []. 2020 None: None. doi: https://doi.org/10.1101/2020.10.21.348912

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