Fraction of trichome area stained for callose (abbreviated as Callose content in the article); determined using built-in functions of Fiji; from binary images generated by thresholding fluorescence and polarized light iphotos using the Yen method as implemented in Fiji software; value is the sum of percentage values from 25 interactively selected detached or in situ trichomes from 3 leaves of 3 plants.
Arabidopsis seeds were sterilized with chlorine gas; stratified in tubes at 4 deg. C for three days in the dark and sown into standard soil substrate pre-treated with Confidor to prevent black flies infestation. Plants were grown under controlled long-day conditions (16 hr light; 23 oC/8 hr darkness; 18 oC; relative air humidity 60 %). Three seedlings per genotype were planted into individual pots at the first true leaf stage. Three approximately fingernail-sized mature; non-senescent rosette leaves per plant were collected at the age of 5 weeks (at which point plants of some but not all of the genotypes were beginning to bolt).
May 17, 2023, 1:48 p.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.