Phenotype: Raffinose 32C
ID:
1910
Study:
AraGWAS Associations:
Scoring:
Soluble carbohydrates were detected using a Waters Acquity UPLC system coupled to a Waters Micromass Quattro Premier triple quadrupole mass spectrometer. Raffonose concentration was measured as nmol/g FW.
Growth conditions:
eeds were sown on agar plates with ½-strength Murashige-Skoog (MS) medium (Duchefa Biochemie B.V., Haarlem, Netherlands), pH 5.7, containing 3% (w/v) sucrose and 1.2% (w/v) phyto agar (Duchefa Biochemie B.V.), and stratified for 7 days at 4 °C in the dark. After stratification plates were transferred to a growth chamber with 9 h/15 h (day/night) photoperiod set to 22 °C and 100-120 µmol m-2 s-1 (white fluorescent light) in a randomised manner and regularly shifted within the growth chamber to avoid any positional effects on seedling establishment and growth. On day 15, seedlings were transferred to a pre-heated growth chamber at 32 °C at Zeitgeber Time (ZT) 2, i.e., two hours after onset of the light period. Control seedlings remained in the 22 °C growth chamber. After four hours seedlings were harvested, flash-frozen in liquid nitrogen and stored at -80 °C until extraction.
Trait-Ontology:
Environment ontology:
Shapiro-Wilk*:
'5.20e-16' (p-value)
Submission date:
June 22, 2023, 8:22 a.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 250 accessions
Phenotype Publications
-
Natural variation of warm temperature-induced raffinose accumulation identifies<i>TREHALOSE-6-PHOSPHATE SYNTHASE 1</i>as a modulator of thermotolerance
[]. 2023 None: None. doi: https://doi.org/10.1101/2023.05.15.540763