Phenotype: Mean(LRD R) IAA
ID:
366
Study:
AraGWAS Associations:
Scoring:
After 3 days of treatment, plates were scanned with CCD flatbed scanners (EPSON Perfection V600 Photo, Seiko Epson, Nagano, Japan), and images used to quantify root parameters with FIJI, by using the tool ‘segmented line’ (Schindelin et al., 2012) as described in Ristova and Busch (2017). In particular, we quantified: primary root length on day 10 (P), growth rate of P after treatment (P2), branching zone or the length of P between the first and last visible lateral root (R), average lateral root length (LRL), and visible lateral root number (LR.No).
Growth conditions:
Seeds were surface sterilized in a desiccator using 100 ml of household bleach and 3.5 ml of 37% HCl for 1 h. The seeds were stratified in water at 4°C in the dark for 3 days, and were sown on square Petri dishes containing 57 ml of growth medium. The control growth medium (before transfer) consisted of one‐fifth‐strength MS medium with MES buffer (Ducheva Biochemie, Haarlem, The Netherlands), 1% sucrose, 0.8% agar (Ducheva Biochemie), and adjusted to pH 5.7.
Trait-Ontology:
Environment ontology:
Shapiro-Wilk*:
'4.84e-08' (p-value)
Submission date:
Sept. 3, 2018, 1:04 p.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 190 accessions
Phenotype Publications
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Natural genetic variation shapes root system responses to phytohormones in Arabidopsis
The Plant Journal. 2018 96: 468-481. doi: 10.1111/tpj.14034