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201 different accessions of A. thaliana from different geographic origins. These accessions were part of the RegMap. Seeds were obtained by growing the parental generation of all lines used side by side in the same growth chambers under the same conditions. The seeds were surface sterilized in 2.8% hypochlorite for 4 min and washed three times in MonoQ sterile water. They were then stratified for 72 h at 4 °C in water and darkness and put to grow on our custom Confocal Chamber System (CCs). The seeds were grown for 3 d in vertically oriented CCs containing 1× Murashige and Skoog (MS) salt mixture, 1% (wt/vol) sucrose and 0.8% (wt/vol) agar. To provide a sterile environment for the CCs and prevent them from drying out, we embedded the CCs into conventional agar plates
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