Phenotype: Leaf Na Mine (μg/g)
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AraGWAS Associations:
Na concentrations in leaves, cultivated in Pb-enriched mine soil. Elemental analysis was performed with an ICP-OES (PerkinElmer).
Growth conditions:
Seeds of the Arabidopsis thaliana HapMap cohort (360 natural populations) were surface sterilized using 3:10 bleach solution in constant agitation for 10 minutes and rinsed with sterile water six times. Seeds were stratified for 4 days at 4 C in 0.1% agar solution to synchronize germination, then 5 seeds per accession were sown in two soil types with contrasted Pb concentrations into pot trays. The control soil was a mix of commercial potting mix soil with perlite (2:1); the mine soil was a mix of soil from an ancient lead mine (Mina de Can Vergeli; 41°53'42.4"N 3°01'11.7"E), commercial potting mix soil and perlite (2:1:1). Trays were placed in a growth chamber with 150 mmol/m2s of light intensity, 12h light/12h dark photoperiod, and 25 ºC /20 ºC day/night temperature. Trays were bottom-watered twice weekly with deionized water. Fifty-day old plants were harvested to measure its nutrient mineral contents.
Environment ontology:
'1.16e-24' (p-value)
Submission date:
July 2, 2021, 8:52 a.m.
* The Shapiro-Wilk test tests the null hypothesis that the data was drawn from a normal distribution.
Geographic distribution of 200 accessions
Phenotype Publications
  • Genome-Wide Association Study Reveals Key Genes for Differential Lead Accumulation and Tolerance in Natural Arabidopsis thaliana Accessions

    Sílvia Busoms, Laura Pérez-Martín, Miquel Llimós, Charlotte Poschenrieder and Soledad Martos

    Frontiers in Plant Science. 2021 12: 1690. doi: 10.3389/fpls.2021.689316

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