Study: Ion Concentration
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DOI
Description
Plants were grown in a controlled environment with 10 h light/ 14 h dark (90 mmol m22s21 photosynthetically active light) and 19 to 22uC, as previously described [31]. Briefly, seeds were sown onto moist soil (Promix; Premier Horticulture) in 10.506210 20 row trays with various elements added to the soil at subtoxic concentrations (As, Cd, Co, Li, Ni, Rb, and Se [31]) and the tray placed at 4uC for 3 days to stratify the seeds and help synchronize germination. Each tray contained 108 plants, six plants each from 18 accessions, with three plants of each accession planted in two different parts of the tray. Each tray contained four common accessions (Col-0, Cvi-0, Fab-2 and Ts-1) used as controls, and 14 test accessions. Trays were bottom-watered twice per week with 0.25-strength Hoagland solution in which Fe was replaced with 10 mM Fe-HBED[N,N9-di(2-hydroxybenzyl)ethyle- nediamine-N,N9-diacetic acid monohydrochloride hydrate; Strem Chemicals, Inc.). After 5 weeks plants were non-destructively sampled by removing one or two leaves and the elemental composition of the tissue analyzed by Inductively Couple Plasma Mass Spectroscopy (ICP-MS). The plant material was rinsed with 18 MV water and placed into Pyrex digestion tubes.
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Publications

Phenotype NameTrait Ontology (TO)Environmental Ontoloy (EO)Unit Ontology (UO)
Zn66zinc concentrationgrowth chamber studyconcentration unit
Se82selenium concentrationgrowth chamber studyconcentration unit
S34sulfur concentrationgrowth chamber studyconcentration unit
Rb85lodicule morphology traitgrowth chamber studyconcentration unit
P31phosphorus concentrationgrowth chamber studyconcentration unit
Ni60nickel concenterationgrowth chamber studyconcentration unit
Na23sodium concentrationgrowth chamber studyconcentration unit
Mo98molybdenum concentrationgrowth chamber studyconcentration unit
Mn55manganese concentrationgrowth chamber studyconcentration unit
Mg25magnesium concentrationgrowth chamber studyconcentration unit
Li7lithium concentrationgrowth chamber studyconcentration unit
K39potassium concentrationgrowth chamber studyconcentration unit
Fe57iron concentrationgrowth chamber studyconcentration unit
Cu65copper concentrationgrowth chamber studyconcentration unit
Co59cobalt concentrationgrowth chamber studyconcentration unit
Cd111cadmium concentrationgrowth chamber studyconcentration unit
Ca43calcium concentrationgrowth chamber studyconcentration unit
B11boron concentrationgrowth chamber studyconcentration unit
As75arsenic concentrationgrowth chamber studyconcentration unit